Kulim KHT Sensors Detection

SENSORS USING LB TECHNIQUE

It is possible to immobilize sensor molecules into LB and LS deposited layers. The sensors can be fabricated from biochemical molecules where proteins, DNA or sugars are the detector molecules and are incorporated in a phospholipid monolayer. Other possibility is to have organic or inorganic dyes, nanoparticles or other functional molecules that give a desired response, and incorporate them into a coating with LB, LS or dip coating into a thin layer on an electrode or other probe. Detection method for such sensors can be electrical, optical or other depending on the sensor application.

An example of immobilized proteins used as sensors:

Immobilization of Alcohol Dehydrogenase in Phospholipid Langmuir-Blodgett Films To Detect Ethanol
Luciano Caseli, Angelo C. Perinotto, Tapani Viitala, Valtencir Zucolotto, and Osvaldo N. Oliveira, Jr.
Langmuir 2009, 25, 3057-3061
Alcohol dehydrogenase (ADH) mixed in dimyristoylphosphatidic acid (DMPA) monolayer exhibited enhanced transfer and detection ability towards ethanol compared to pure ADH layers. Studies proved that the ADH structure remained unchanged over one month in the mixed monolayer under storage conditions. A sensor array deposited on gold electrodes could detect alcohol down to 10 ppb concentration. For more details of the study please check the original publication.