In situ investigations on enzymatic degradation of poly

SPR Bionavis

Q-Sense 4 sample in one Run workhorse

The present study leads to the following conclusion. QCM-D

and SPR can be used to investigate the enzymatic degradation of

a polymer film in real time, where the changes in mass and morphology

of the film can be well described by the frequency and

dissipation in QCM-D as well as the SPR signal response. PCL

with small crystallites degrades in a way of layer-by-layer with

pseudo first-order degradation kinetics. The degradation of PCL

with crystalline lamellae exhibits two-stage kinetics, which relates

to the degradation of amorphous PCL and PCL crystallites,

respectively. The former degrades more quickly than the latter,

leading the film to be microporous

Surface Plasmon Resonance (2 Channels/exchangeable cuvette)

BB Band played superbly that night ..... Impressed .... Especially Jorma...

Advantages of SPR NAVI 200

• Increased angular range that enables simultaneous gas and liquid-phase measurements

• Contamination-free index matching with a proprietary optical gel

• “Drop-in” sensor plate design• Integrated high-performance liquid handling

• Dual measurement channels for in-line referencing and/or duplicate measurements

• Support for a second laser wave length, for simultaneous determination of RI and thickness

• Automated determination of measurement parameters for sensogram measurement

• High-accuracy sensogram (kinetic) measurements• Easy use of user-defined/modified surfaces

Application notes are provided at our web site: www.bionavis.com

Kulim Kht DP Meeting Helsinki

An Introduction to Biophysical Characterisation

using Dual Polarisation Interferometry

Dual Polarisation Interferometry (DPI) is a highly versatile, powerful analytical technique for

biophysical characterisation of proteins and other biomolecules. It extends the typical dynamic

measurements of conventional biosensors by including an additional quantitative, sub molecular,

conformational measurement.

DPI delivers a unique perspective on biochemistry, linking conformational changes to biochemical activity at a resolution normally associated with ‘big physics.

Two polarisations of light are passed through the

sensor which consists of an upper ‘sensing’ layer

and an embedded ‘reference’ layer. The output

from each of the layers then combine producing two

interference patterns which are detected by a

camera.

As molecules bind (or change shape) on the

surface of the ‘sensing’ layer, they are probed by

the evanescent field from the ‘sensing’ surface.

This, in turn, changes both interference patterns. As

material binds, the interference pattern shifts one

way. As material is removed from the surface, the

interference pattern returns.

The AnaLight® Resolver software automatically analyses both sets of data and outputs the real time

changes in the thickness, density and mass of the molecular layer. All of the calculations are based on

classical optics theory and have been independently validated by, amongst others, the National

Physics Laboratory (NPL) in the UK. www.farfield-group.com

Goniometer Kulim Kht KSV